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Source Molecular Corporation |
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Professional Dedicated Service
Fast Turnaround Time
Multiple Host Detection Offered |
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About Source Molecular Corporation
Source Molecular Corporation is a team of scientists and business professionals with expertise in genetic and molecular sciences. The company is dedicated to the field of microbial source tracking using the most advanced genomic and molecular techniques. Source Molecular is dedicated to helping improve water quality and has analyzed thousands of water samples throughout North America, making it one of the leaders in the microbial source tracking industry. A stringent effort is made to provide only the most current and peer reviewed technology and tests as MST is a constantly evolving field with continuous advancements.
Services Available
At present, Source Molecular offers tests that can identify and quantify fecal contamination from 12 host sources -- Human, Cattle, Swine, Gull, Goose, Chicken, Dog, Deer, Elk, Horse, Bird and Ruminant (see below).
Clients wishing to identify fecal pollution sources will receive unlimited assistance in crafting appropriate sampling and MST plans. Shipping kits, including sterile sample bottles, are available so that clients are prepared to sample contaminated waters as soon as the opportunity arises, such as after a rain event. The Source Molecular shipping kit is also recommended for quality assurance and quality control purposes. Please see our detailed packing instructions for more information.
Upon sample arrival and analysis, Source Molecular delivers the results of MST tests in 5-10 working days, one of the fastest turnaround times in the industry. Clients may opt for presence/absence testing or quantification testing to determine the concentration of the host-associated genetic markers in the water samples. See our sample presence/absence report and our sample quantification report. Source Molecular recommends conducting two or more tests per water sample in order to strengthen the validity of the results, particularly negative results. In addition, Source Molecular suggests sampling during both dry and wet events.
To discuss project pricing and design, please email or call Source Molecular at (786) 220-0379.
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| Fecal Pollution Toolbox |
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| Tests |
Target |
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| Human Fecal Bacteria: Bacteroides dorei |
| Human Fecal Bacteria: Bacteroides stercoris |
| Human Fecal Bacteria: Bacteroides spp. |
| Human Fecal Bacteria: Bacteroides fragilis |
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| Human Fecal Virus: Polyomavirus |
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| Tests |
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| Cattle Fecal Bacteria: Bacteroidetes |
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| Cattle Fecal Virus: Enterovirus |
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| Swine Fecal Bacteria: Bacteroidetes |
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| Bird Fecal Bacteria: Helicobacter |
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| Gull Fecal Bacteria: Catellicoccus |
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| Test |
Target |
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| Goose Fecal Bacteria: Bacteroidetes |
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| Chicken Fecal Bacteria: Bacteroidetes |
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| Dog Fecal Bacteria: Bacteroidetes |
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| Tests |
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| Deer Fecal Bacteria: Enterococcus |
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| Elk Fecal Bacteria: Bacteroidetes |
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| Ruminant Fecal Bacteria: Bacteroidetes |
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| Test |
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| Horse Fecal Bacteroidetes |
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Quality Control and Accreditation
Quality control is maintained with the latest scientific laboratory equipment and highly trained, degreed personnel. Source Molecular uses scientifically rigorous (i.e. published and peer-reviewed) molecular analytical methods that enable it to identify with high confidence any contamination, which lead to false positives. The possibility of qPCR inhibition, a common cause of false negative results, is also monitored.
Source Molecular is on its way to becoming ISO/IEC 17025:2005 accredited. It received its pending accreditation notice from the American Association for Laboratory Accreditation (A2LA) indicating that the accreditation process is set to be finalized later this year. Upon completion, Source Molecular will be provided a Certificate and Scope of Accreditation detailing the tests for which the company has been judged competent to perform. This will make Source Molecular the only dedicated Microbial Source Tracking laboratory in the country to be ISO/IEC 17025:2005 accredited and serves as a recognition of its laboratory's quality and high standards.
Collaborators and Research Partners
Source Molecular has collaborated with various government agencies, private organizations and academic research groups to develop new and validate existing MST assays. Collaborators include:
• The U.S. Environmental Protection Agency's National Risk Management Research Laboratory (Cincinnati, OH)
• The National Oceanic and Atmospheric Administration (Miami, FL)
• Southern California Coastal Water Research Project (Costa Mesa, CA)
• Delaware Department of Natural Resources & Environmental Control (Dover, DE)
• The University of Miami (Miami, FL)
• Nova Southeastern University (Ft. Lauderdale, FL)
• University of California, Los Angeles (Los Angeles, CA)
• New Mexico State University (Las Cruces, NM)
• University of South Florida (Tampa, FL)
Research Projects
The following are sample research projects that Source Molecular was or is currently involved in:
Microbial Source Tracking in Johnson Creek, 2012 – Source Molecular assisted the Johnson Creek Watershed Council in determining the potential source polluting Johnson Creek in Oregon where one third of water samples exceeded E.coli state standards. Water samples were analyzed for human-associated and bird-associated bacteria. The study showed potential human fecal contamination and appropriate remediation efforts are underway.
Microbial Source Tracking Interlaboratory Method Evaluation Study, 2011 – The study was conducted by the Southern California Coastal Water Research Project (SCCWRP) to challenge and compare approximately 45 well tested and novel fecal source tracking methods. Source Molecular took part in validating a human-associated method as well as in comparing traditional vs. quantitative PCR assays. Top performing methods will be used to conduct MST in California. Results from the study will be published in a special issue of Water Research dedicated to MST.
Captiva Water Quality Assessment Project, 2010 – Source Molecular took part in the last phase of the Captiva Water Quality Assessment Project conducted by the Sanibel-Captiva Conservation Foundation Marine Laboratory. The SCCF Marine Laboratory sought Source Molecular's services in differentiating sources of fecal bacteria, especially Enterococci. See: 2010 Project Approval Letter
Recent Peer Reviewed Publications
- B.A. Layton, Y. Cao, D.L. Ebentier, K. Hanley, E. Balleste, et al. 2012. Performance of human fecal anaerobe-associated PCR-based assays in a multi-laboratory method evaluation study. Manuscript submitted for publication.
- T.E. Riedel, A.G. Zimmer-Faust, V. Thulsiraj, T. Madi, K.T. Hanley, et al. 2012. Comparison of detection limits and costs of conventional and quantitative PCR assays targeting human or sea gull-associated markers in artificial and environmental waters. Manuscript submitted for publication.
- H.M. Solo-Gabriele, A.B. Boehm, T.M. Scott, and C.D. Sinigalliano. 2011. Beaches and coastal environments. In C. Hagedorn, A.R. Blanch, V.J. Harwood (eds). Microbial Source Tracking: Methods, Applications, and Case Studies. Springer. New York, NY
- C.D. Sinigalliano, J.M. Fleisher, M.L. Gidley, H.M. Solo-Gabriele, T. Shibata, et al. 2010. Traditional and molecular analyses for fecal indicator bacteria in non-point source subtropical recreational marine waters. Water Research 44: 3763-3772.
- T.M. Scott, V.J. Harwood, W. Ahmed, Y. Masago, and J.B. Rose. 2009. Comment on “environmental occurrence of the enterococcal surface protein (esp) gene is an unreliable indicator of human fecal contamination. Environmental Science & Technology 43: 6434-6435.
Reports
The following are examples of reports describing projects that Source Molecular was involved in:
- Michigan DEQ contracted Source Molecular to conduct bacterial source tracking on various sites in the Ecorse and Detroit River watersheds. qPCR-based microbial source tracking was used to accurately detect the presence of Human fecal contamination. The results allowed Michigan DEQ to identify combined sewer overflows and/or sanitary sewer overflows as potential sources of their high fecal indicator bacteria. See: 2007 Project Report
- As part of the City of Jacksonville's LSJR Tributary Assessment Project, Source Molecular analyzed blind fecal samples sent by the University of South Florida. Source Molecular correctly identified all blind fecal samples sent within the 48-hour hold time requirements. Microbial source tracking assisted the City of Jacksonville in determining the sources and locations of fecal contamination. See: 2008-2009 Project Report
- The Texas Commission on Environmental Quality approved Source Molecular involvement in the TMDL Implementation of Upper San Antonio River, Salado Creek and Walzem Creek. Source Molecular was tapped by the San Antonio River Authority to conduct microbial source tracking analyses to quantify the presence or absence of bacteria from human sources. Source Molecular used microbial source tracking to isolate and identify Human genetic markers. See: 2008 TMDL Upper San Antonio River
- Whitefish Lake Institute in Montana asked Source Molecular to provide microbial source tracking in its investigation of the spatial and temporal extent of septic leachate to the shoreline area of Whitefish Lake. Using library-independent qPCR technology, Source Molecular's contribution allowed the Institute to better understand the sources contributing to the fecal contamination in their watershed. See: 2012 Project Report
- The Delaware Department of Natural Resources and Environmental Control (DNREC) sought Source Molecular and Dr. Jody Harwood’s assistance in determining the source of high Enterococci levels observed at Tower Road Bayside Beach. Water samples were analyzed for dog, human and seagull fecal indicator bacteria. The study results show seagulls to be consistent, major contributors to the fecal contamination at this beach.
Humphries, E. 2011. Microbial Source Tracking at Tower Road Bayside Beach, Rehoboth Bay, Sussex County, Delaware, 2011.
Presentations
- Mauricio Larenas. Microbial Source Tracking: Search for Human, Gull, and Dog. 12th Annual Great Lakes Beach Association Conference held on October 16-18, 2012 on Mackinac Island, MI. Download Microbial Source Tracking – Great Lakes 2012
- Mauricio Larenas. In Search of Gulls: A MST Case Study at Delaware’s Tower Road Beach. The GOMA MST Workshop III on Microbial Source Tracking, Pathogen Indicators, and Rapid Methods held on April 9-11, 2012 in St. Petersburg, FL. Download GOMA Microbial Source Tracking Presentation
- Mauricio Larenas. Microbial Source Tracking Process and Applications. US EPA Region 4 Beach Managers Meeting held on March 20-21, 2012 in Biloxi, MS. Download Microbial Source Tracking Presentation
- C. Sinigalliano, D. Wanless, N. Lucey, T. Scott, H. Solo-Gabriele, et al. Source Tracking for Public Health Protection: Quantitative Molecular Detection of Gull and Canine Fecal Contamination in Recreational Waters and Beaches. 2010 Northern Gulf Institute Annual Meeting held in 2010 in Mobile, AL Poster. See the poster here.
- Troy Scott. Microbial Source Tracking: Applications and Field Studies. Michigan State University Pathogen Workshop Series – Microbial Source Tracking held on March 16, 2007 in East Lansing, MI.
Contact
Source Molecular Corporation
4985 SW 74th Court
Miami, Florida 33155
Tel: (786) 220-0379
Email:
info@sourcemolecular.com
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